In our cohort, both the Ob and MASLD groups had elevated insulin resistance compared to the NW group, but several biomarkers (ALT, AST, NEFA, triglycerides, CK‐18, CK‐18 fragments, collagen IV, miR‐122 and miR‐192) were higher in the MASLD group compared to the Ob group, highlighting that insulin resistance is not a distinguishing feature of MASLD in our study. This evidence concerns the gene GPT and metabolic dysfunction-associated steatotic liver disease.