Sertraline exhibited potent cytotoxicity against triple‐negative breast cancerReduced viability by 98.07% at 10 μM, comparable to doxorubicinInduced apoptosis and caused cell cycle arrestSuppressed colony formation and angiogenesisModulated key cancer‐related genes (VEGF, P53, P21, ZEB1, HSP70). Here, TP53 is linked to cancer.