In addition, as antigen peptides for the MHC‐I molecules are generally 8–10 amino acid residues long, pVIII N‐terminus display is suitable for displaying CD8+ T cell epitopes for cancer vaccine applications.[35] We constructed a helper plasmid, rEES, containing a recombinant pVIII (rpVIII) under the control of a Tac promoter (Figure3A, second row) to express the SIINFEKL epitope with the peptide sequence of EESIINFEKL. The gene discussed is CD8A; the disease is cancer.