NFKB1 and viral infectious disease: Through CRISPR-Cas9 knockout screening, we identified the positive selection genes, which were primarily implicated in biological processes such as amino acid and metal ion transport, as well as cellular responses to sterols, while the negative selection genes were mainly involved in the regulation of the NIK/NF-κB signaling pathway and cytoskeletal and membrane components, which are crucial for the cell’s defense mechanisms against viral infection [26].