Thus, the cumulative suppression of proliferation, IL-6/IL-8 production, migration/invasion, and AKT/mTOR signaling activity is characterized by high biological and methodological robustness, with the stability of results across alternative dispersion models and exclusion of potentially atypical experiments attesting to the validity of the findings regarding the anti-inflammatory potential of targeted PI3K/AKT/mTOR inhibition in RA-FLSs. This evidence concerns the gene AKT1 and rheumatoid arthritis.