To investigate the surface expression of ICMs on immune cells, we used flow cytometry to measure the frequencies of CD3+ CD4+ and CD3+ CD8+ T-cell subsets expressing TIM-3, TIGIT, CTLA-4, LAG-3 and PD-1 in the PBMCs isolated from HIV (HIV+ mono-infected patients n = 15), HIV-TB (HIV-Mtb co-infected patients n = 10), TB (Mtb + mono-infected patients n = 15), and Control (healthy volunteers n = 15). Here, CTLA4 is linked to tuberculosis.