Meanwhile, the TF saturation was significantly higher in the young (92.99 ± 1.68% vs. 26.70 ± 8.18%, p < 0.001) and old iron overload groups (94.13 ± 0.98% vs. 20.58 ± 3.89%, p < 0.001) compared with their respective control groups (Figure 1D). This evidence concerns the gene TF and Tangier disease.