Abe et al. used focused ultrasound at 1.5 MHz, 2% dc, 200 W, and a prf of 5 Hz, for 20 s treatments in a HER2-transduced preclinical murine breast cancer model to induce mechanical damage with or without the addition of anti-PDL1 (given intraperitoneally 3 and 6 days after the ultrasound treatment), and investigated the transcription of genes 8 days after exposures using single-cell transcriptomics [32]. The gene discussed is CD274; the disease is breast carcinoma.