Further techniques in cell lines revealed that TGFb1 enhances L1CAM expression in a Slug-dependent manner and that this upregulation led to a phenotype of epithelial-to-mesenchymal transition, which would be in accordance with their results in the endometrial cancer tissue specimens, where more L1CAM expression was associated with less estrogen receptor (ER), PR, and E-cadherin expression [21]. This evidence concerns the gene ESR1 and endometrial cancer.