In addition, a study on the host immune response induced by C. perfringens infection in pig ileum indicated that, unlike the jejunum, C. perfringens was able to activate the TLR4/MyD88/NF-κB signalling pathway in the ileum, activating downstream immune-related cytokines [35,47]. This evidence concerns the gene TLR4 and infection due to clostridium perfringens.