Aiming to identify the effect of autophagy on PXR expression, three mtKRAS and three mtBRAF CRC cell lines (DLD-1, HCT116, and SW480 and RKO, colo-205, and HT29, respectively) were exposed to two autophagy inhibitors (5 mM of 3-MA and 10 mM of HCQ) and a dual inhibitor of AKT/mTOR (1μΜ of PI-103) for 24 h. The gene discussed is AKT1; the disease is colorectal carcinoma.