Due to the activation of autophagy in mtKRAS CRC cell lines (HCT116, DLD-1, and SW480) after being treated with Irinotecan and to explore the mechanisms by which both PXR expression and autophagy are increased and activated, we treated all three mtKRAS cell lines (HCT116, DLD-1, and SW480) with 10 μΜ of Irinotecan after silencing PXR expression with 80 nM of siRNA. This evidence concerns the gene NR1I2 and colorectal carcinoma.