Moreover, both sirtuins’ levels were lower in the myocardium of old mice than in young animals, and acute myocardial ischemia followed by reperfusion injury led to further decreases in the sirtuins levels in both age groups, accompanied by mitochondrial relocation of SIRT1 that resulted in SIRT3-related protein mobilization [82]. This evidence concerns the gene SIRT1 and myocardial ischemia.