We detected the levels of VEGFA and its upstream transcription factor HIF-1α in the cortex, and the results showed that combined AS and TMP could significantly improve the reduction in VEGFA expression levels and the rise of HIF-1α, suggesting HIF-1α and VEGF may be involved in the mechanism of combined AS and TMP in improving cerebral ischemia. Here, HIF1A is linked to Cerebral ischemia.