Many preclinical studies have also shown that the lack of TIMP-1 exacerbates BBB damage in animals with focal cerebral ischemia [24,25], and the ratio of MMP-9 to TIMP-1 is relevant to BBB injuries and water content in the brain than these two factors alone [26,27], while therapeutic interventions focused on TIMP-1 and its downstream signals may be valuable in the improvement of BBB function after brain injury and neurological diseases [28]. This evidence concerns the gene TIMP1 and nervous system disorder.