CsA treatment led to- significant elevation in IFNL2/3 mRNA.- decreased MERS-CoV viral load.- improved expression of the epithelial integrity marker SCNN1B.- There was also a significant reduction in lung pathology and interstitial inflammation compared to DMSO control.- Upon analysis on day seven post-infection, an inverse correlation was noted between IFNL expression and MERS-CoV levels in the lung homogenates.- The comprehensive data illustrate that the oral administration of CsA stimulates IFNλ synthesis in the pulmonary system of mice, thereby exerting significant anti-viral properties. This evidence concerns the gene SCNN1B and infection.