RAB10 and infection: When ubiquitination was inhibited at the time of infection with 15 μM PYR-41 (0 hpi), no nuclear staining of pIE1 was observed (Figure 4B,C), the Golgi structure and location were altered (Figure 4B,E), without expansion of Rab10 TMs (Figure 4B,D), suggesting that PYR-41 prevents pre-AC formation, consistent with the observation that PYR-41 at 0 hpi blocks infection and prevents viral gene expression.