A previous study demonstrated that in vivo treatment with two (E)-2-(1-(4-chlorophenylthio) propan-2-ylidene)-hydrazine carbothioamides prolonged the survival of T. gondii-infected wild-type mice, and this was associated with increased IFN-γ and IL-12 production [37], highlighting the potential of this chemical class as promising therapeutic candidates for toxoplasmosis treatment. This evidence concerns the gene IFNG and toxoplasmosis.