TNF and obesity due to melanocortin 4 receptor deficiency: As shown in Figure 1, under ME conditions in vitro, we detected in BC cells significantly increased expression of TNFα, CCL2, IL-6 and human IL-8, well-known TLR-controlled cytokines, tightly implicated in breast tumorigenesis [35,36,37,38,39,40,41] and the obesity-cancer link [6,42,43,44,45].