Critical methodological constraints merit acknowledgment: Primarily, although VCL, PIM1, CDK6, and SNHG3 showed significant associations with AML survival and prognosis, they did not exhibit pathological differential expression in qRT-PCR experimental validations; thus, mechanistic interrogation through single-cell level functional perturbation assays (e.g., using siRNA or small-molecule inhibitors) remains imperative to delineate precise pathobiological interactions within this regulatory axis. The gene discussed is PIM1; the disease is acute myeloid leukemia.