As shown in Figure 4D,E, PICRUSt2 showed that CA supplementation enriched (p < 0.05) the substance dependence signaling pathway at the Level 2 KEGG pathway, and mineral absorption, biosynthesis of various secondary metabolites-part 3, cocaine addiction, serotonergic synapse, dopaminergic synapse, amphetamine addiction, alcoholism, thiamine metabolism, and thyroid hormone signaling pathway at the Level 3 KEGG pathway. The gene discussed is TG; the disease is substance dependence.