Quantitative PCR analysis revealed that the expression levels of ASK1, TRADD, JUN, MKK7, MKK4, JNK2, and JNK3 were significantly upregulated in GCs from AF compared to HF (Figure 3A–G), confirming activation of the ASK1 signaling pathway during follicular atresia. This evidence concerns the gene MAP3K5 and hydrops fetalis.