While Yang et al. demonstrated DAA-mediated suppression of TNFα/IL-1β in macrophages via NF-κB inhibition [36], our paradoxical findings in AD rats may be due to the following reasons: First, Tissue-specific effects: neuronal/glial methylation dynamics may differ from peripheral macrophages, a view supported by previous research [37]. The gene discussed is NFKB1; the disease is Alzheimer disease.