To further clarify whether cynaroside attenuates hepatocyte senescence in NASH-T2DM by modulating HMGCS2-regulated ketone body production, we used adeno-associated virus (AAV8)-shHMGCS2 to treat the mouse model of NASH + T2DM (Figure 7A). This evidence concerns the gene HMGCS2 and metabolic dysfunction-associated steatohepatitis.