Boyden assays (48 wells), three per cell line, for two rat astrocytoma cell lines (C6 and F98) and five human glioblastoma cell lines (T98G, LN18, U87, LN229, and U373), showed cell migration to HGF alone as a chemoattractant (row 2), HGF and FBS together as chemoattractants (rows 3–9), and to FBS alone as a chemoattractant in a dose curve (rows 10–12), compared to background (unstimulated) cell migration (row 1). The gene discussed is HGF; the disease is glioblastoma.