Because tumor spheroid formation is sensitive to serum levels and medium composition [38], we tested low-serum conditions and methylcellulose (MC) supplementation to minimize external variability and support anchorage-independent growth [39], with the aim of confirming that the observed effects in B16 cells were specifically attributable to CCRL2 expression, and not confounded by culture conditions. The gene discussed is CCRL2; the disease is neoplasm.