To note, in an in vitro study we have previously observed that NRTI modulates NLRP3-related genes differently (PYCARD, caspase-1, IL-18 and IL-1β), and miR-223-3p and miR-7-1-5p expression in monocytes of AD and HC individuals, measured by ddPCR [58], suggest that the regulatory mechanisms are different in relation of the disease state. This evidence concerns the gene PYCARD and Alzheimer disease.