In myocardial infarction-related I/R models, S-nitrosylation of Trx, PAF, GRK2, CypD, and the ND3 subunit of mitochondrial complex I reduces infarct size and protects the heart from I/R injury, while S-nitrosylation of sEH exacerbates I/R damage. Here, PCLAF is linked to myocardial infarction.