CD274 and neoplasm: Immunofluorescence and DSP of tumor biopsies further uncovered spatial heterogeneity within the TME: macrophage activation and T cell infiltration were observed in both PD-L1-positive and PD-L1-negative tumors, with significant downregulation of ECM-related genes in PD-L1-negative tumors, suggesting that the treatment potentially reversed the immunosuppressive TME.