Supplementary Figure S6a,b shows that FSP1 siRNA was effective in knocking down FSP1 expression in HEK293 and a panel of ovarian cancer cells (PEO1, PEO4 and OVCAR3). In PEO1, PEO4, and OVCAR3 cells, a tolerable dose of RSL3 (30–50 nM) was used for each cell line. Cell viability assays were carried out to compare cells with and without FSP1 knockdown. FSP1 knockdown markedly sensitized these ovarian cell lines to RSL3-induced ferroptosis (Figure 4a–c). The gene discussed is AIFM2; the disease is ovarian cancer.