Here, we established a pipeline of hepatic microvascular imaging through HD-fMOST to provide high-resolution quantitative anatomical data on the characteristics and architecture of liver vasculature in wild-type (WT) mice and Akt/Ras HCC mouse models, which has significant implications for the further development of new tumor vasculature-targeting therapies. Here, AKT1 is linked to hepatocellular carcinoma.