All four genes were found to have very high sensitivity and specificity values, which were 92.5% and 91.6% (area under curve (AUC) = 0.97) for Collagen type IV alpha 2 chain (COL4A2), 88.8% and 88% (AUC = 0.97) for Collagen type IV alpha 1 chain (COL4A1), 88.8% and 96.4% (AUC = 0.96) for T-cell leukemia homeobox 2 (TLX2), and 82.5% and 96.4% (AUC = 0.95) for Integrin Subunit Alpha 4 (ITGA4) in distinguishing stool samples from CRC patients from healthy individuals. Here, COL4A1 is linked to colorectal carcinoma.