Expanding the previous research indicating the significance of the EGFR and IGF-IR pathways in MDA-MB-231, as well as the diminished motility following treatment with either an EGFR or IGF-IR inhibitor in MCF-7 2D cell cultures [23], we employed 3D models to enhance our comprehension of the migratory potential of these two breast cancer cell lines in a setting that simulated that of in vivo tumors. This evidence concerns the gene IGF1R and breast carcinoma.