This study was designed to evaluate two primary research objectives: first, to emphasize the advantages of 3D models in replicating the structure and function of breast tumors under pathological conditions, and second, to investigate the roles of RTKs—specifically EGFR and IGF-IR—in cellular behavior and modified ECM expression across two breast cancer subtypes: TNBC (MDA-MB-231) and luminal A (MCF-7). Here, EGFR is linked to breast carcinoma.