This research is therefore focused on two primary aims: (i) to illustrate the enhanced biological relevance of 3D cell culture models in mimicking the features of the TME compared to that of the conventional 2D systems and (ii) to evaluate the roles of EGFR and IGF-IR in the behavior of breast cancer cells and the expression of major MMPs using the respective downstream inhibitors, AG1478 and AG1024. The gene discussed is EGFR; the disease is breast carcinoma.