Finally, we included 10 well-known cancer-related genes (or their hotspot-containing exons) listed as most frequently mutated in lung cancer (BRAF,EGFR,ERBB2,HRAS,KRAS,MAP2K1,MET,NF1,NRAS, and RIT1) [37] in the panel to serve as internal controls for highly mutated regions (Fig. 1A). The gene discussed is HRAS; the disease is lung cancer.