HMGB2 and melanoma: To directly test the role of HMGB2 in melanoma invasion, we used in vitro invasion assays (Fig. 5e) and found that targeting HMGB2 with siRNA significantly impaired invasion (P = 0.0133; Fig. 5f,g), whereas overexpression of HMGB2 increased invasion (P = 0.0180; Fig. 5h,i).