CD274 and cancer: High-level enrichment of CD8+ T lymphocytes within tumor tissues predicts a better patient prognosis.20 However, cancer cells induce the dysfunction of CD8+ T lymphocytes through various mechanisms, thereby creating a suppressive TIME.21 For example, a previous study reported that hexokinase domain component 1 (HKDC1) weakens the tumor-clearance activity of cytotoxic T lymphocytes through activating the STAT1/PD-L1 axis in tumor cells.22 On the basis of our scRNA-seq findings, we hypothesized that TACC3 shapes a negative TIME in a manner dependent on CD8+ T cells.