Next, flow cytometry analysis of tumor tissues from an in situ HCC model revealed that interference with TACC3 expression in Hepa1‒6 cells increased the ratio of total tumor-infiltrating CD8+ T cells and IFN-γ+CD8+ T cells while clearly decreasing the proportion of PD-1+CD8+ T cells (Fig. 3d, e and Supplementary Fig. 10a). This evidence concerns the gene CD8A and hepatocellular carcinoma.