Based on this, this study established a rat model of middle cerebral artery occlusion/reperfusion (MCAO/R)-induced CIRI to systematically investigate the protective effect of AS-IV on ischemic-reperfusion brain injury and its molecular mechanism in mediating mitophagy through the ROS-PINK1/Parkin pathway, aiming to provide experimental and theoretical support for its clinical application in the treatment of ischemic stroke. Here, PRKN is linked to ischemic stroke.