TNF and steatosis: Network analyses also revealed upregulated inflammatory responses and JNK signaling in KD-TN, including Tnf, Il-1b, Il-6, Ccl21, Mapk13, Tlr4, and Traf1. Notably, the elevated expression of Col1a1 and Anxa2 in KD-TN suggests enhanced ECM deposition that may initiate fibrosis, along with increased Nfkb2 and Krt8, which accelerate inflammation and cellular remodeling, key steps in the progression of steatosis to MASH.