All analyzed TLRs (TLR2, TLR3, TLR4, TLR7, TLR8, and TLR9) exhibited high sensitivity and specificity in the diagnosis of endometriosis, suggesting that their expression on CD4+, CD8+, and CD19+ lymphocytes may serve as a potential marker for differentiating patients with endometriosis from healthy women. Here, TLR9 is linked to endometriosis.