Both confluent and proliferating HepaFH3 cells (HepaFH3 C and HepaFH3 P) showed a statistically significant reduction in albumin, hepatocyte nuclear factor 4 alpha (HNF4A), and cytochrome P450 3A4 (CYP3A4) expression compared to non-HCC-PHHs isolated from tumor-free liver tissue. Here, HNF4A is linked to hepatocellular carcinoma.