Transcript levels of classic hepatocyte markers, albumin and HNF4A, were significantly reduced in HepaFH3 cells, and CYP3A4 was expressed equally low in both HepaFH3 and HCLs, consistent with previously reported downregulation of hepatic differentiation markers in both HCC and proliferating in vitro models [50,54,55]. This evidence concerns the gene HNF4A and hepatocellular carcinoma.