Our findings indicate that FN inhibits DIRAS3-induced autophagy in ovarian cancer cells, as evidenced by reduced conversion of LC3I to LC3II on Western blots, decreased LC3 puncta observed by fluorescence staining, increased p62 expression, and impaired autophagic flux as demonstrated by mCherry-LC3 reporter analysis. This evidence concerns the gene DIRAS3 and ovarian carcinoma.