In this study, the intestinal inflammation model induced by DSS exhibited typical pathological features: substantial accumulation of ROS, abnormal aggregation of neutrophils in the intestine, obvious intestinal tissue damage, reduced secretion of acidic mucin, significantly increased expression levels of the pro-inflammatory factor IL-6 and immune factors IL-10 and IL-12, and activation of the TLR4/NF-κB signaling pathway, ultimately leading to the development of intestinal inflammation in zebrafish. This evidence concerns the gene NFKB1 and inflammation.