Dual blockade or gene knockout of LAG-3 and PD-1 produced high levels of IL-2, IFN-γ, TNF-α, and granzyme B, increased the percentage of CD8+ and CD4+ TILs, enhanced the effector function of CD8+ T cells, reduced the frequency of suppressive Tregs within the TME so that it delayed tumor growth and extended the life span of mice significantly 130. The gene discussed is CD4; the disease is neoplasm.