Dual blockade or gene knockout of LAG-3 and PD-1 produced high levels of IL-2, IFN-γ, TNF-α, and granzyme B, increased the percentage of CD8+ and CD4+ TILs, enhanced the effector function of CD8+ T cells, reduced the frequency of suppressive Tregs within the TME so that it delayed tumor growth and extended the life span of mice significantly 130. This evidence concerns the gene TNF and neoplasm.