To further reveal the specificity and potential importance of NDUFS1 in pulmonary epithelial cells, we performed single-cell data mining analysis on LPS-induced ALI mice (GSE276682) and identified that NDUFS1 was mainly enriched in epithelial cells and macrophages, with the expression of NDUFS1 decreased in epithelial cells with LPS stimulation (Fig.1C). This evidence concerns the gene NDUFS1 and acute respiratory distress syndrome.