We next treated PLK4-overexpressing NB cells with or without pathway-specific inhibitors-LY294002 (PI3K/Akt, 5 μM), BIRB796 (p38 MAPK, 20 μM), and PD98059 (MEK/ERK, 100 μM)-for 48 hours. Here, AKT1 is linked to neuroblastoma.