Compared with control cells, JCAD-overexpressing (JCAD-OE) cells presented greater proliferation, colony formation, and EdU-positive cell ratios whereas JCAD-knockdown (JCAD-KD) cells presented the opposite trend (Fig. 3A-D), confirming the role of JCAD in promoting breast cancer cell proliferation. This evidence concerns the gene JCAD and breast carcinoma.