SEPTIN9 and neoplasm: Given our previous reports that the NAT10/SEPT9/HIF-1α positive feedback loop governs glycolysis addiction and that HIF-1α can also foster VEGFA-associated angiogenesis within the hypoxic tumor microenvironment11, 31, we detected the expression of VEGFA mRNA in three groups of gene deficient GC cell lines (shNAT10, shYAP1 and shHIF1A GC cells) under both normoxic and hypoxic (100 μM CoCl2) conditions by qPCR.