Finally, we compared the mutation profiles between PT and OM using the DESeq2 and identified seven mutated genes from the OM sites (P <0.05), including FLCN, DNAJC13, DSC3, SLC9A3, ADGRV1, SCAPER and ARHGEF12 (Figure 2E). The gene discussed is ARHGEF12; the disease is ocular melanoma.