To further confirm these results, we stained for other astrocyte markers, including S100β, ALDH1L1 and Vimentin, and found that the density of S100β+ (Figure 4B, G), ALDH1L1+ (Figure 4C, H) and Vimentin+ (Figure 4D, I) astrocytes was significantly decreased in the motor cortex of YAPGFAP-CKO ALS mice, suggesting that the density of astrocytes was reduced in the motor cortex of YAPGFAP-CKO ALS mice. Here, ALDH1L1 is linked to amyotrophic lateral sclerosis.