Notably, both Nissl staining and immunostaining of NeuN showed a significant reduction in the density of Nissl bodies (Figure 2A-B) and NeuN+ neurons (Figure 2C-D) in the motor cortex of YAPGFAP-CKO ALS mice, compared to YAPf/f ALS mice. This evidence concerns the gene RBFOX3 and amyotrophic lateral sclerosis.