To further investigate the molecular mechanism of astrocytic YAP in C9orf72-poly-GA ALS, we collected CM from N2a cells transfected with GFP-GA5 plasmid and GFP-GA100 plasmid, described as GA5 CM and GA100 CM, respectively, and treated YAP+/+ and YAP-/- astrocytes with them (Supplementary Figure S3F). This evidence concerns the gene C9orf72 and amyotrophic lateral sclerosis.