We observed elevated percentages of CD11b+ Ly6G+ neutrophils, CD11b+ Ly6G- Ly6C+ monocytes, CD8+ CTLs, and activated CTLs (CD8+ CD69+), as well as decreased percentage of M2 macrophages upon chitosan treatment (Figure 2M-P, Supplemental Figure S3D); indicative of an activation of the tumor immune microenvironment. This evidence concerns the gene CD69 and neoplasm.