Established asciminib-resistant (ASCI-R) clones of KCL-22 cell line (n = 8; Supplementary Methods) were analyzed using Next Generation Sequencing (NGS) to identify somatic mutations in SH3, SH2, and kinase (KD) domains of BCR::ABL1 and in 62 selected leukemia-associated genes [5]. The gene discussed is ABL1; the disease is leukemia.