In this study, we used a therapeutic strategy by blocking the CCL2-CCR2 axis using an intratracheal instillation of an antagonist of CCR2 (CCR2-Ant) or of a blocking antibody against CCL2 (CCL2-Ab) for treating ALI previously triggered by a double-hit model induced by HCl/LPS. The gene discussed is CCL2; the disease is acute respiratory distress syndrome.